Reversible Loss of the Potassium Effect in Distilled Water

Not only does distilled water take away the irritability of Nitella but it also changes its behavior toward potassium. In normal cells potassium is strongly negative to sodium but after sufficient exposure to distilled water this effect disappears. It can be restored by returning the cells to their normal environment or to a suitable nutrient solution. This change in the protoplasm seems to be chiefly in its outer surface.